Prophylactic Potential of Tender Coconut Water on Haematology Disorder in Benzene-Induced Lymphoid Malignancy in Wister Rats

Background and Objective: Tender coconut water has been associated with various health and medicinal benefits. Therefore, the aim of this study was to evaluatethe prophylactic potential of tender coconut water on haematology disorder in benzene-induced lymphoid malignancy in rats in order to ascertain the potential therapeutic effect of coconut water in these disease conditions. Materials and Methods: Eighteen Wistar rats (170-200 g) were divided into 3 groups, namely: Negative control, positive control and phytopreventive with each group consisting of six rats. Pretreatment with 10, 20 and 30 mL kg^–1 body weight of fresh Malayan green dwarf hybridized tender coconut water daily for 4 weeks before benzene-induced lymphoid malignancy 48 hourly for 4 weeks. Complete blood counts for haematological parameters were determined by Sysmex haematology autoanalyzer XP-300 (Kobe, Japan). Results: The total white blood cells, lymphocytes and platelets were significantly higher in the positive control compared to the negative control and phytopreventive group (p<0.05). The haematocrit value, haemoglobin value, red blood cell count and neutrophils were also significantly lower in positive control than the negative control and phytopreventive group (p<0.01, 0.05) while different white cell types and red blood cell indices were not significant (p>0.05). Induction of lymphoid malignancy in Wistar rats was successful. However, phytopreventive shows restoration of normal histology of the lymphatic organs without expression of the p53 gene and Bcl-2 except 20 and 30 mL kg^–1 doses in the spleen that showed vacuities indicating 10 mL kg^–1 body weight doses as preferable. Conclusion: This study has shown that benzene induced lymphoid malignancy is associated with the haematological disorder but was prevented with Malayan green dwarf hybridized tender coconut water with 10 mL kg^–1 body weight daily. This has provided a unique approach to lymphoid malignant prevention.